Journal: Protein expression and purification
Article Title: Expression and purification of the mitochondrial transmembrane protein FAM210A in Escherichia coli
doi: 10.1016/j.pep.2023.106322
Figure Lengend Snippet: (A) Purification of full length FAM210A fusion protein from BL21(DE3) cells using a 0.25 mM IPTG induction for 3.5 hr at 37°C. Lane 1: Protein marker ladder. Lane 2: Uninduced cells. Lane 3: Induced cells. Lane 4: Cell debris pellet. Lane 5: Cell lysate flowthrough. Lane 6: Cell lysate supernatant. Lane 7: Cell lysate wash. Lane 8: Elution 1. Lane 9: Elution 2. Lane 10: Elution 3. Lane 11: Solubilized membrane flowthrough. Lane 12: Solubilized membrane wash. Lane 13: Membrane elution 1. Lane 14: Membrane elution 2. (B) Purification of dMTS FAM210A fusion protein using BL21(DE3) cells (100 mL) with 0.25 mM IPTG induction for 3.5 hr at 37°C. * is placed at the solubilized FAM210A fusion protein that was extracted from bacterial membranes. Lane 1: Protein marker ladder. Lane 2: Uninduced cells. Lane 3: Induced cells. Lane 4: Cell debris pellet. Lane 5: Cell lysate flowthrough. Lane 6: Cell lysate supernatant. Lane 7: Cell lysate wash. Lane 8: Elution 1. Lane 9: Elution 2. Lane 10: Elution 3. Lane 11: Solubilized membrane flowthrough. Lane 12: Solubilized membrane wash. Lane 13: Membrane Elution 1. Lane 14: Membrane elution 2.
Article Snippet: Affinity purification by nickel-based immobilized metal affinity chromatography The solution of bound resin was transferred to a gravity flow purification column (Bio-Rad, Cat. # 7311550).
Techniques: Purification, Marker, Membrane